期刊
TRENDS IN BIOCHEMICAL SCIENCES
卷 38, 期 1, 页码 30-37出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibs.2012.10.005
关键词
single-molecule fluorescence; macromolecular complex; cell lysate; single-molecule immunoprecipitation; single-molecule pull-down; protein
资金
- European Research Council [ERC-StG-2012-309509]
- Fondation pour la Recherche Medicale
- Research Center Program of IBS (Institute for Basic Science) [EM1202]
- National Honor Scientist Program through the National Research Foundation of Korea (NRF)
Single-molecule fluorescence spectroscopy offers real-time, nanometer-resolution information. Over the past two decades, this emerging single-molecule technique has been rapidly adopted to investigate the structural dynamics and biological functions of proteins. Despite this remarkable achievement, single-molecule fluorescence techniques must be extended to macromolecular protein complexes that are physiologically more relevant for functional studies. In this review, we present recent major breakthroughs for investigating protein complexes within cell extracts using single-molecule fluorescence. We outline the challenges, future prospects and potential applications of these new single-molecule fluorescence techniques in biological and clinical research.
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