期刊
TREE PHYSIOLOGY
卷 30, 期 11, 页码 1415-1432出版社
OXFORD UNIV PRESS
DOI: 10.1093/treephys/tpq082
关键词
air pollution; biochemistry; difference in gel electrophoresis (DiGE); photosynthesis; primary carbon metabolism; proteomics
类别
资金
- Fonds National de la Recherche Luxembourg (FNR) [AFR TR-PHD BFR06-044]
Populus tremula L. x Populus alba L. (Populusxcanescens (Aiton) Smith) - clone INRA 717-1-B4 saplings (50 cm apex to base and carrying 19 leaves on average) - were followed for 28 days. Half of the trees were grown in charcoal-filtered air while the other half were exposed to 120 ppb ozone for 11 h a day during the light period. The expanding leaf number 4 was tagged at the beginning of the experiment and finished expansion between 7 and 14 days. These leaves were harvested weekly for biochemical and proteome analyses using quantitative bidimensional electrophoresis (DiGE). Independent of the ozone treatment, all the analyses allowed a distinction between expanding and adult leaves. The results indicate that during the expansion phase (Days 0-7) the enzymatic machinery of the leaves is set up, and remains dynamically stable in the adult leaves (Days 14-28). Although ozone had no apparent effect on expanding leaves, the metabolic stability in fully expanded leaves observed in ozone-free plants was disturbed after 2 weeks of exposure and a stress-induced response became apparent.
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