期刊
TREE PHYSIOLOGY
卷 29, 期 10, 页码 1299-1305出版社
OXFORD UNIV PRESS
DOI: 10.1093/treephys/tpp054
关键词
fatty acid; palmitate
类别
资金
- Chinese Academy of Sciences [KSCX2-YW-G-035, KSCX2-YW-G-027]
- NSFC-Guangdong Joint Fund of Natural Sciences [U0733005]
- CAS/SAFEA International Partnership Program for Creative Research Teams
- CAS '100 Talents' program
A full-length cDNA of an acyl-acyl carrier protein (ACP) thioesterase (TE) (EC 3.1.2.14), named JcFATB1, was isolated from the woody oil plant Jatropha curcas L. The deduced amino acid sequence of the cDNA shares about 78% identity with FATB TEs, but only about 33% identity with FATA TEs from other plants. The deduced sequence also contains two essential residues (H-317 and C-352) for TE catalytic activity and a putative chloroplast transit peptide at the N-terminal. Southern blot analysis revealed that a single copy of JcFATB1 is present in the J. curcas genome, and semi-quantitative PCR analysis showed that JcFATB1 was expressed in all tissues that were examined, most strongly in seeds, in which its expression peaked in late developmental stages. Seed-specific overexpression of the JcFATB1 cDNA in Arabidopsis resulted in increased levels of saturated fatty acids, especially palmitate, and in reduced levels of unsaturated fatty acids. The findings suggest that JcFATB1 from this woody oil plant can function as a saturated acyl-ACP TE and could potentially modify the seed oil of J. curcas to increase its levels of palmitate.
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