期刊
TREE GENETICS & GENOMES
卷 9, 期 5, 页码 1247-1256出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11295-013-0632-x
关键词
Interphase chromatin; Chromocenters; DNA methylation; Epigenetics; Histone acetylation; Histone methylation; Quercus robur
资金
- Croatian Ministry of Science, Education and Sport [119-1191196-1224]
- AUF (Agence Universitaire de Francophonie) PSCI Grant
Cycling cells of Quercus robur have a simple nuclear organization where most of the heterochromatin is visible as DAPI-positive chromocenters, which correspond to DAPI bands at the (peri)centromeric region of each of the 24 chromosomes of the oak complement. Immunofluorescence using 5-mC revealed dispersed distribution of the signal throughout the interphase nucleus/chromosomes without enrichment within DAPI-positive chromocenters/bands, suggesting that DNA methylation was not restricted to constitutive heterochromatin, but was associated with both euchromatic and heterochromatic domains. While H3K9ac exhibited typical euchromatin-specific distribution, the distributional pattern of histone methylation marks H3K9me1, H3K27me2, and H3K4me3 showed some specificity. The H3K9me1 and H3K27me2, both heterochromatin-associated marks, were not restricted to chromocenters, but showed additional dispersed distribution within euchromatin, while H3K27me2 mark also clustered in foci that did not co-localize with chromocenters. Surprisingly, even though H3K4me3 was distributed in the entire chromatin, many chromocenters were enriched with this euchromatin-specific modification. We discuss the distribution of the epigenetic marks in the context of the genome composition and lifestyle of Q. robur.
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