期刊
STEM CELLS TRANSLATIONAL MEDICINE
卷 4, 期 5, 页码 513-522出版社
OXFORD UNIV PRESS
DOI: 10.5966/sctm.2014-0267
关键词
Exosomes; Angiogenesis; Regenerative medicine; Wnt4; beta-Catenin
资金
- National Natural Science Foundation of China [31340040, 81272481, 81270214]
- Jiangsu Province for Outstanding Sci-tech Innovation Team in Colleges and Universities [SJK2013-10]
- Jiangsu Province's Outstanding Medical Academic Leader and Sci-tech Innovation Team Program [coproduct 201117]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Jiangsu Province's Doctoral Innovation Fund [CXZZ13_0703]
Human umbilical cord mesenchymal stem cells (hucMSCs) and their exosomes have been considered as potential therapeutic tools for tissue regeneration; however, the underlying mechanisms are still not well understood. In this study, we isolated and characterized the exosomes from hucMSCs (hucMSC-Ex) and demonstrated that hucMSC-Ex promoted the proliferation, migration, and tube formation of endothelial cells in a dose-dependent manner. Furthermore, we demonstrated that hucMSC-Ex promoted wound healing and angiogenesis in vivo by using a rat skin burn model. We discovered that hucMSC-Ex promoted beta-catenin nuclear translocation and induced the increased expression of proliferating cell nuclear antigen, cyclin D3, N-cadherin, and beta-catenin and the decreased expression of E-cadherin. The activation of Wnt/beta-catenin is critical in the induction of angiogenesis by hucMSC-Ex, which could be reversed by beta-catenin inhibitor ICG-001. Wnt4 was delivered by hucMSC-Ex, and the knockdown of Wnt4 in hucMSC-Ex abrogated beta-catenin nuclear translocation in endothelial cells. The in vivo proangiogenic effects were also inhibited by interference of Wnt4 expression in hucMSC-Ex. Taken together, these results suggest that hucMSC-Ex-mediated Wnt4 induces beta-catenin activation in endothelial cells and exerts proangiogenic effects, which could be an important mechanism for cutaneous wound healing.
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