期刊
TRANSLATIONAL ONCOLOGY
卷 6, 期 2, 页码 124-132出版社
ELSEVIER SCIENCE INC
DOI: 10.1593/tlo.12268
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资金
- H.W. & J. Hector Foundation [M39.1]
- Federal Ministry of Education and Research (BMBF) [0315-452-C]
- Deutsche Krebshilfe/Dr Mildred-Scheel-Stiftung [10-2089-FI 1]
- Alfried Krupp von Bohlen und Halbach Foundation (Award for Young Full Professors, Essen, Germany)
- Hella-Buhler-Foundation (Heidelberg, Germany)
- Dr Ingrid zu Solms Foundation (Frankfurt/Main, Germany)
- Walter Schulz Foundation (Munich, Germany)
- Deutsche Krebshilfe (Bonn, Germany)
- German-Israeli Cooperation DKFZ-MOST
- Wilhelm Sander Foundation (Munich, Germany)
BACKGROUND: Signaling through stromal cell-derived factor-1 alpha (SDF-1 alpha), strongly secreted by bone marrow stromal cells and the CXC chemokine receptor 4 (CXCR4) exposed on tumor cells has pivotal roles in proliferation, metastasis, and tumor cell dormancy. Dormancy is associated with cytostatic drug resistance and is probably a property of tumor stem cells and minimal residual disease. Thus, hampering the SDF-1 alpha/CXCR4 cross talk by a CXCR4 antagonist like Plerixafor (AMD3100) should overcome tumor cell dormancy by mobilization of tumor cells from sanctuary niches. Our aim was to elucidate the direct effects exerted by SDF-1 alpha and Plerixafor on proliferation, chemosensitivity, and apoptosis of CXCR4-expressing tumor cells. METHODS: The ability of SDF-1 alpha and Plerixafor to regulate intracellular signaling, proliferation, and invasion was investigated using two colon cancer cell lines (HT-29 and SW480) with either high endogenous or lentiviral expression of CXCR4 compared to their respective low CXCR4-expressing counterparts as a model system. Efficacy of Plerixafor on sensitivity of these cell lines against 5-fluorouracil, irinotecan, or oxaliplatin was determined in a cell viability assay as well as stroma-dependent cytotoxicity and apoptosis assays. RESULTS: SDF-1 alpha increased proliferation, invasion, and ERK signaling of endogenously and lentivirally CXCR4-expressing cells. Exposure to Plerixafor reduced proliferation, invasion, and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling. Combination of chemotherapy with Plerixafor showed an additive effect on chemosensitivity and apoptosis in CXCR4-overexpressing cells. An SDF-1-secreting feeder layer provided a protective niche for CXCR4-overexpressing cells resulting in decreased chemosensitivity. CONCLUSION: CXCR4-antagonistic therapy mobilizes and additionally sensitizes tumor cells toward cytoreductive chemotherapy.
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