期刊
TRANSGENIC RESEARCH
卷 23, 期 5, 页码 757-765出版社
SPRINGER
DOI: 10.1007/s11248-014-9813-0
关键词
Embryonic stem (ES) cell; Y chromosome; Gene-targeting
类别
资金
- Grants-in-Aid for Scientific Research [24613006] Funding Source: KAKEN
We developed a transgenic mouse line with Y chromosome-linked green fluorescent protein expressing transgenes (Y-GFP) by the conventional microinjection into the pronucleus of C57BL/6J fertilized oocytes. Embryonic stem (ES) cells derived from Y-GFP mice enabled not only sexing but also the identification of 39, XO karyotype by the lack of Y chromosome. Actually, when fluorescence activated cell sorting (FACS) was applied to Y-GFP ES cells, non-fluorescent ES cells were conveniently collected and showed the lack of Y chromosome by PCR genotyping and Southern blot analysis. FACS analysis revealed Y chromosome loss occurred at 2.9 % of 40, XY ES cells after five passages. These Y-GFP ES cells are potentially applicable to reduce the time, cost and effort needed to generate the gene-targeted mice by the production of male and female mice derived from the same ES cell clone.
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