期刊
TRANSGENIC RESEARCH
卷 22, 期 6, 页码 1241-1247出版社
SPRINGER
DOI: 10.1007/s11248-013-9711-x
关键词
Transgenic mice; Bacterial artificial clone; LacZ; Basal ganglia
类别
资金
- Moritani Scholarship Foundation
- JSPS KAKENHI [20500301, 20220007]
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [20500301] Funding Source: KAKEN
We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked chloramphenicol acetyltransferase gene, and examined the Cre activity in the Tg mouse brains by assessing beta-galactosidase activity. Cre activity was specifically detected in the caudate-putamen, nucleus accumbens, and olfactory tubercle of the Gpr88-Cre Tg mouse brain. Medium spiny neurons within the caudate-putamen exhibited Cre activity. Thus, Gpr88-Cre Tg mice could be a useful tool for analyzing the function of the basal ganglia by using Cre/loxP systems.
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