期刊
TRANSGENIC RESEARCH
卷 22, 期 4, 页码 805-812出版社
SPRINGER
DOI: 10.1007/s11248-012-9682-3
关键词
beta-Glucanase; Parotid gland; Transgenic mice
类别
资金
- National Basic Research Program of China (973 Program) [2011CB944200]
- Chinese transgenic animal project [2011ZX 08007-003, 2011ZX08006-004]
The feasibility of using the pig parotid secretory protein promoter to drive the beta-glucanase transgene expression in mouse parotid glands was examined in this study. The parotid gland-specific vector expressing beta-glucanase gene (GLU, from Paenibacillus polymyxa CP7) was constructed. Transgenic mice were produced by the pronuclear microinjection. Both PCR and Southern blot analysis showed that the mice carried the beta-glucanase gene and the beta-glucanase gene could be stably inherited. Furthermore, RT-PCR and northern blot analysis indicated that it was specifically expressed in the parotid. The beta-glucanase activity in the saliva was found to be 0.18 U/mL. After feeding a diet containing 2 % beta-glucan, the average daily gain of transgenic was significantly higher than non-transgenic mice. The crude protein and crude fat concentration in faeces of transgenic mice were significantly reduced compared with that of the non-transgenic mice. These results suggest that the successful expression of foreign beta-glucanase in the animal parotid would offer a promising biological approach to reduce the anti-nutritional effect of beta-glucans in feed.
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