期刊
TRANSGENIC RESEARCH
卷 22, 期 1, 页码 195-200出版社
SPRINGER
DOI: 10.1007/s11248-012-9635-x
关键词
ES cells; C57BL/6N; Transgenic mice; EGFP; ICSI
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Grants-in-Aid for Scientific Research [20670006, 24700430, 21112006, 20062008] Funding Source: KAKEN
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
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