期刊
TRANSGENIC RESEARCH
卷 22, 期 2, 页码 359-368出版社
SPRINGER
DOI: 10.1007/s11248-012-9652-9
关键词
Cotton; Disease resistance; NPR1; Seedling disease; Thielaviopsis basicola; Transgenic
类别
资金
- Cotton Inc.
- Texas Higher Education Coordinating Board-Advanced Research Program [000517-0005-2006]
- Texas AgriLife Research
Black root rot, caused by Thielaviopsis basicola, is an important disease in several crops including cotton. We studied the response of Arabidopsis NPR1 (AtNPR1)-expressing cotton lines, previously shown to be highly resistant to a diverse set of pathogens, to a challenge from T. basicola. In four different experiments, we found significant degree of tolerance in the transgenic lines to black root rot. Although transformants showed the typical root discoloration symptoms similar to the wild-type control plants following infection, their roots tended to recover faster and resumed normal growth. Better performance of transgenic plants is reflected by the fact that they have significantly higher shoot and root mass, longer shoot length, and greater number of boll-set. Transcriptional analysis of the defense response showed that the roots of AtNPR1-overexpressing transgenic plants exhibited stronger and faster induction of most of these defense-related genes, particularly PR1, thaumatin, glucanase, LOX1, and chitinase. The results obtained in this investigation provide further support for a broad-spectrum nature of the resistance conferred by overexpression of AtNPR1 gene in cotton.
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