期刊
TRANSGENIC RESEARCH
卷 20, 期 1, 页码 177-181出版社
SPRINGER
DOI: 10.1007/s11248-010-9393-6
关键词
Vitamin E; Tocopherols; Metabolic engineering; Transgenic maize
类别
资金
- Ministerio de Ciencia e Innovacion, Spain [BFU2007-61413, BES-2005-9161]
- BIOFORCE ERC
- ICREA Funding Source: Custom
The quantity and composition of tocopherols (compounds with vitamin E activity) vary widely among different plant species reflecting the expression, activity and substrate specificity of enzymes in the corresponding metabolic pathway. Two Arabidopsis cDNA clones corresponding to rho-hydroxyphenylpyruvate dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol methyltransferase (MPBQ MT) were constitutively expressed in corn to further characterize the pathway and increase the kernel tocopherol content. Transgenic kernels contained up to 3 times as much gamma-tocopherol as their wild type counterparts whereas other tocopherol isomers remained undetectable. Biofortification by metabolic engineering offers a sustainable alternative to vitamin E supplementation for the improvement of human health.
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