期刊
TRANSGENIC RESEARCH
卷 19, 期 1, 页码 105-112出版社
SPRINGER
DOI: 10.1007/s11248-009-9288-6
关键词
Balb/c; eGFP; Lentiviral transgenesis; Bone-marrow chimeric mouse
类别
资金
- Hungarian Academy of Sciences
- [OTKA T 049034]
- [OMFB-00118/2008]
- [OTKA PD 78310]
Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors eGFP expression was driven by the human EF1 alpha promoter. The established transgenic animals were analyzed for eGFP expression by in vivo fluorescence imaging, PCR, histology and flow-cytometry. eGFP expression showed even distribution without mosaicism; however, tissue-dependent differences of eGFP expression were observed. Up to the sixth generation only one newborn showed eGFP inactivation. eGFP + transgenic bone marrow cells efficiently provided long-term haemopoietic repopulation in radiation chimeras, regenerating all bone marrow-derived lineages with eGFP + cells with distinct eGFP expression profiles. The established eGFP + BALB/c mouse strain is expected to be extremely useful in various immunological experiments.
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