期刊
TRANSGENIC RESEARCH
卷 18, 期 6, 页码 899-909出版社
SPRINGER
DOI: 10.1007/s11248-009-9277-9
关键词
Bioassay; 17 beta-Estradiol; Estrogenic compounds; Estrogen receptors; 4-Nonylphenol; Transgenic plants
类别
资金
- Bio-oriented Technology Research Advancement Institution (BRAIN)
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [17208029]
- Research Institute of Innovative Technology for the Earth (RITE)
- Grants-in-Aid for Scientific Research [17208029] Funding Source: KAKEN
Transgenic Arabidopsis plants carrying a recombinant human estrogen receptor gene and a green fluorescent protein reporter gene were used to bioassay estrogenic compounds. We constructed four recombinant human estrogen receptor genes by combining the DNA-binding domain of LexA, a synthetic nuclear localization signal, a ligand-binding domain of the human estrogen receptor, and a transactivation domain of VP16 in different orders; the XEV plants were the most sensitive, and were able to detect 0.001 ng ml(-1) of 17-estradiol (E-2). The transgenic plants absorbed E-2 and 4-nonylphenol present in the nutrient solution, whereas most of the other compounds seemed to be retained in, or on, the roots. Estrone, methoxychlor, bisphenol A, 4-nonylphenol, and 4-t-octylphenol in the medium were clearly detected by RT-PCR and PCR of the genomic DNA. The transgenic Arabidopsis XEV plants thus have potential for the bioassay of estrogenic compounds.
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