期刊
TRANSGENIC RESEARCH
卷 18, 期 1, 页码 59-69出版社
SPRINGER
DOI: 10.1007/s11248-008-9196-1
关键词
DmAMP1; Magnaporthe oryzae; Rhizoctonia solani; Transgenic rice; Plant-microbe interactions; Defensins
类别
资金
- Department of Biotechnology
- Ministry of Science and Technology, Government of India
Magnaporthe oryzae and Rhizoctonia solani, are among the most important pathogens of rice, severely limiting its productivity. Dm-AMP1, an antifungal plant defensin from Dahlia merckii, was expressed in rice (Oryza sativa L. sp. indica cv. Pusa basmati 1) using Agrobacterium tumefaciens-mediated transformation. Expression levels of Dm-AMP1 ranged from 0.43% to 0.57% of total soluble protein in transgenic plants. It was observed that constitutive expression of Dm-AMP1 suppresses the growth of M. oryzae and R. solani by 84% and 72%, respectively. Transgenic expression of Dm-AMP1 was not accompanied by an induction of pathogenesis-related (PR) gene expression, indicating that the expression of DmAMP1 directly inhibits the pathogen. The results of in vitro, in planta and microscopic analyses suggest that Dm-AMP1 expression has the potential to provide broad-spectrum disease resistance in rice.
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