期刊
TRANSGENIC RESEARCH
卷 17, 期 5, 页码 985-989出版社
SPRINGER
DOI: 10.1007/s11248-008-9199-y
关键词
BiFC; binary vector; fluorescent protein; FRET; pDH51; pGreen; GATEWAY
类别
资金
- Biotechnology and Biological Sciences Research Council
Fluorescent protein labelling technologies enable dynamic protein actions to be imaged in living cells and can also be used in conjunction with other methods such as Forster resonance energy transfer and biomolecular fluorescence complementation. In this report, we describe the generation of a series of 23 novel GATEWAY-compatible vectors based on pGreenII and pDH51 backbones with the latest fluorescent protein tags (Cerulean, EGFP and Venus) and the choice of three in planta selection markers. These vectors can be obtained from the Nottingham Arabidopsis Stock Centre (N9819-N9846) and should be a powerful tool box for transgenic research in plants.
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