期刊
TRAFFIC
卷 13, 期 7, 页码 960-978出版社
WILEY
DOI: 10.1111/j.1600-0854.2012.01356.x
关键词
fission; fusion; mitochondria; organelle dynamics; peroxisome
类别
资金
- German Research Foundation (DFG) [SCHR 518/6-1, SCHR 518/6-2]
- Conselho de Reitores das Universidades Portuguesas and Deutsche Akademische Austauschdienst (CRUP/DAAD) [A-20/08]
- Portuguese Foundation for Science and Technology (FCT) [REEQ/1023/BIO/2005, PTDC/BIA-BCM/71932/2006, PTDC/SAU-OSM/103647/2008, PEst-C/CTM/LA0025/2011, SFRH/BD/37647/2007]
- University of Aveiro
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-OSM/103647/2008, PTDC/BIA-BCM/71932/2006, SFRH/BD/37647/2007] Funding Source: FCT
Peroxisomes and mitochondria show a much closer interrelationship than previously anticipated. They cooperate in the metabolism of fatty acids and reactive oxygen species, but also share components of their fission machinery. If peroxisomes - like mitochondria - also fuse in mammalian cells is a matter of debate and was not yet systematically investigated. To examine potential peroxisomal fusion and interactions in mammalian cells, we established an in vivo fusion assay based on hybridoma formation by cell fusion. Fluorescence microscopy in time course experiments revealed a merge of different peroxisomal markers in fused cells. However, live cell imaging revealed that peroxisomes were engaged in transient and long-term contacts, without exchanging matrix or membrane markers. Computational analysis showed that transient peroxisomal interactions are complex and can potentially contribute to the homogenization of the peroxisomal compartment. However, peroxisomal interactions do not increase after fatty acid or H2O2 treatment. Additionally, we provide the first evidence that mitochondrial fusion proteins do not localize to peroxisomes. We conclude that mammalian peroxisomes do not fuse with each other in a mechanism similar to mitochondria! fusion. However, they show an extensive degree of interaction, the implication of which is discussed.
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