期刊
TRAFFIC
卷 12, 期 8, 页码 1000-1011出版社
WILEY
DOI: 10.1111/j.1600-0854.2011.01209.x
关键词
intracellular transport; RNA imaging; RNA regulation; RNA transport; single-particle tracking; temporal analysis
类别
资金
- Georgia Tech Administration
- NIH [EB009455, GM094198]
Transport of messenger RNAs (mRNAs) in the cytoplasm is essential for localization to translation sites and for post-transcriptional regulation. Utilizing single-RNA sensitive probes and real-time fluorescence microscopy, we accurately quantified the dynamics of native, non-engineered, beta-actin mRNAs within the cytoplasm of epithelial cells and fibroblasts for the first time. Using single-particle tracking and temporal analysis, we determined that native beta-actin mRNAs, under physiologic conditions, exhibit bursts of intermittent, processive motion on microtubules, interspersed between time periods of diffusive motion, characterized by non-thermal enhanced diffusivity. When transport processes were perturbed via ATP depletion, temperature reduction, dynamitin overexpression and chemical inhibitors, processive motion was diminished or eliminated and diffusivity was reduced. These data support a model whereby processive, motor-driven motion is responsible for long-distance mRNA transport.
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