期刊
TRAFFIC
卷 10, 期 7, 页码 938-950出版社
WILEY
DOI: 10.1111/j.1600-0854.2009.00909.x
关键词
endosome; nerve growth factor; organelle fractionation; receptor; signal transduction
类别
资金
- Whitehall Foundation
- NIH [R15NS061303]
- COBRE NCRR [P20 RR015583]
- Health Research Council, Cancer Society
- Neurological Foundation
- Lottery Health
- Lottery Science
- Real Kids Trust
- Palmerston North Medical Research Foundation
- Massey University
- NIH BRIN NCRR [PR-16455-02]
Receptor endocytosis is regulated by ligand binding, and receptors may signal after endocytosis in signaling endosomes. We hypothesized that signaling endosomes containing different types of receptors may be distinct from one another and have different physical characteristics. To test this hypothesis, we developed a high-resolution organelle fractionation method based on mass and density, optimized to resolve endosomes from other organelles. Three different types of receptors undergoing ligand-induced endocytosis were localized predominately in endosomes that were resolved from one another using this method. Endosomes containing activated receptor tyrosine kinases (RTKs), TrkA and EGFR, were similar to one another. Endosomes containing p75(NTR) (in the tumor necrosis receptor superfamily) and PAC1 (a G-protein-coupled receptor) were distinct from each other and from RTK endosomes. Receptor-specific endosomes may direct the intracellular location and duration of signal transduction pathways to dictate response to signals and determine cell fate.
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