期刊
TRAFFIC
卷 10, 期 2, 页码 186-200出版社
WILEY
DOI: 10.1111/j.1600-0854.2008.00857.x
关键词
Bst1p; COPII; Cwp2p; Emp24p; ER exit site; Erv14p; GPI-anchored proteins; Hxt1p; Per1p
类别
资金
- Human Frontiers Science Program Organization through a long-term fellowship
- National Sciences and Engineering Research Council of Canada through a postdoctoral fellowship
- German Academic Exchange Service through a post-doctoral fellowship
- Swiss National Science Foundation through a Marie Heim-Vogtlin fellowship
Previous biochemical work has revealed two parallel routes of exit from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae, one seemingly specific for glycosyl-phosphatidylinositol (GPI)-anchored proteins. Using the coat protein II (COPII) mutant sec31-1, we visualized ER exit sites (ERES) and identified three distinct ERES populations in vivo. One contains glycosylated pro-alpha-factor, the second contains the GPI-anchored proteins Cwp2p, Ccw14p and Tos6p and the third is enriched with the hexose transporter, Hxt1p. Concentration of GPI-anchored proteins prior to budding requires anchor remodeling, and Hxt1p incorporation into ERES requires the COPII components Sec12p and Sec16p. Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p.
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