期刊
TRAFFIC
卷 9, 期 6, 页码 936-950出版社
WILEY
DOI: 10.1111/j.1600-0854.2008.00735.x
关键词
batten; btn1; CLN3; end4; endocytosis; F-actin; myo1; neuronal ceroid lipofuscinosis; polarity; sla2; sterol; Sz. pombe
类别
资金
- Medical Research Council Funding Source: Medline
- Wellcome Trust Funding Source: Medline
btn1, the Schizosaccharomyces pombe orthologue of the human Batten disease gene CLN3, exerts multiple cellular effects. As well as a role in vacuole pH homoeostasis, we now show that Btn1p is essential for growth at high temperatures. Its absence results in progressive defects at 37 degrees C that culminate in total depolarized growth and cell lysis. These defects are preceded by a progressive failure to correctly polarize sterol-rich domains after cytokinesis and are accompanied by loss of Myo1p localization. Furthermore, we found that in Sz. pombe, sterol spreading is linked to defective formation/polarization of F-actin patches and disruption of endocytosis and that these processes are aberrant in btn1 Delta cells. Consistent with a role for Btn1p in polarized growth, Btn1p has an altered location at 37 degrees C and is retained in actin-dependent endomembrane structures near the cell poles or septum.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据