期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 109, 期 -, 页码 1-18出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2018.09.013
关键词
Thioflavin T; Sensor; Fluorescence; G-quadruplex; Label-free
资金
- National Natural Science Foundation of China [21205142]
- State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University [2017006]
- Research Innovation Program for Graduates of Central South University [2018zzts384, 2018zzts399]
Thioflavin T (ThT), a water-soluble fluorescence probe and the conventional dye for the detection of amyloid fibrils has recently been demonstrated to recognize and bind nucleic acids. It induces the formation of G-quadruplex (G4) structure and appears as a sensor by emitting enhanced fluorescence. Incorporation of G-rich sequences in the nucleic acid amplification procedures assists the observation of the final products. In this manner, ThT with the light-up nature has emerged as a well-liked dye being devised in several biosensing applications. It offers sensitive and straightforward detection of bio-substrates including nucleic acids, proteins, small molecules as well as measurement of enzyme activities with the advantages of specificity, reduced background signal, and minimal cost. In this review, we aim to present a thorough overview of the ThT-based biosensing applications along with ThT/G-quadruplex complex formation and ThT-associated amplification techniques. (c) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据