期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 63, 期 -, 页码 21-30出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2014.06.019
关键词
Automated method development; Column/mobile-phase screening; HPLC/UHPLC method conversion; Method development; Method optimization; Method transfer; Pharmaceutical analysis; Quality control; Stability-indicating method; Ultra-high-pressure liquid chromatography (UHPLC)
In recent years, ultra-high-pressure liquid chromatography (UHPLC) became the modern standard HPLC platform. UHPLC, with its shorter analysis time and quicker column equilibration, is ideally suited to rapid method development. This article provides a critical review of the current status, the benefits and the limitations of UHPLC in method development. We use case studies to describe best practices and recent advances. Examples include conversion of existing HPLC methods to faster analysis, rapid column/mobilephase screening, and automated method optimization. While we focus on the development of reversedphase methods for assay and impurity analysis of small-molecule pharmaceuticals, our insights and conclusions can be extended to other applications and sample types. Besides generating faster analysis (when used with short, small-particle columns), the higher pressure limits of UHPLC also allow the effective use of longer columns for superior routine analysis of complex samples. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据