期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 30, 期 10, 页码 1587-1597出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2011.08.006
关键词
Aptamer; Bacteria; Bioanalytical assay; Biosensor; DNA; In-vitro selection; Pathogen; Protein; SELEX; SOMAmer
资金
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program
- Alberta Health and Wellness
- Alberta Water Research Institute
- Canadian Water Network
DNA aptamers specifically recognizing microbial cells and viruses have a range of analytical and therapeutic applications. This article describes recent advances in the development of aptamers targeting specific pathogens (e.g., live bacteria, whole viral particles, and virally-infected mammalian cells). Specific aptamers against pathogens have been used as affinity reagents to develop sandwich assays, to label and to image cells, to bind with cells for flow-cytometry analysis, and to act as probes for development of whole-cell biosensors. Future applications of aptamers to pathogens will benefit from recent advances in improved selection and new aptamers containing modified nucleotides, particularly slow off-rate modified aptamers (SOMAmers). (C) 2011 Elsevier Ltd. All rights reserved.
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