期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 28, 期 7, 页码 854-864出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2009.03.002
关键词
Enzyme immobilization; Mass spectrometry; Protease; Protein analysis; Protein digestion; Proteolytic enzyme; Proteomics; Trypsin microreactor; Trypsin nanoreactor; Tryptic digestion
资金
- European Union FP6 COBRED [LSHB-CT-2007-037730]
- Austrian Ministry of Arts, Science and Education [GZ 200.065/2-VI/2/2002]
Protein digestion utilizing proteases (e.g., trypsin, Lys C and other proteolytic enzymes) is one of the key sample-preparation steps in contemporary proteomics, followed by liquid chromatography coupled to mass spectrometry (MS). Tryptic digestion is traditionally performed in aqueous solutions, usually applying the enzyme and the sample in a 50:1 protein-to-protease ratio. Long digestion times (up to 24 h), auto-digestion sub-products and poor enzyme-to-substrate ratio are common issues with liquid-phase protein-digestion processes. The use of enzymes immobilized onto solid supports can minimize these problems by increasing enzyme-to-substrate ratios, significantly speeding up digestion times and reducing autolysis. The other main goal of protease immobilization is to obtain rugged, efficient enzyme reactors. In this article, we review the most important proteolytic enzyme-immobilization techniques with the main emphasis on fabrication of trypsin microreactors and nanoreactors and their utilization in bottom-up proteomics. We also discuss data reportedly obtained using the various immobilization protocols with respect to enzyme activity and MS-sequence coverage. (C) 2009 Elsevier Ltd. All rights reserved.
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