期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 27, 期 3, 页码 205-214出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2007.11.003
关键词
electrospray ionization; ion mobility spectroscopy; liquid chromatography; mass spectrometry; metabolomics
资金
- NCI NIH HHS [R21 CA126191, R33 CA126191, R21 CA126191-01] Funding Source: Medline
- NCRR NIH HHS [P41 RR018522-05, P41 RR018522] Funding Source: Medline
- NIDDK NIH HHS [R21 DK071283, R33 DK071283, R33 DK071283-03] Funding Source: Medline
- NIEHS NIH HHS [R01 ES022190] Funding Source: Medline
The goal of metabolomics analyses is detection and quantitation of as many sample components as reasonably possible in order to identify compounds or features that can be used to characterize the samples under study. When utilizing electrospray ionization to produce ions for analysis by mass spectrometry (MS), it is important that sample constituents are efficiently separated prior to ion production, in order to reduce complexity and minimize ionization suppression and thereby extend the dynamic range of the measurement, as well as the coverage of the metabolome. Similarly, optimization of the MS inlet and interface can lead to increased measurement sensitivity. This review focuses on the role of high-resolution liquid chromatography (LC) separations in conjunction with improved ion production and transmission for LC-MS-based metabolomics. We place additional emphasis on the compromise between metabolome coverage and sample-analysis throughput. (c) 2007 Elsevier Ltd. All rights reserved.
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