期刊
TOXICON
卷 90, 期 -, 页码 326-336出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2014.08.064
关键词
Diet; Tityus serrulatus; Scorpion feeding; Venom composition; Hyaluronidase; Ontogenetic variation
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, The State of Sao Paulo Research Foundation, scholarship) [2012/12954-6, 2012/13590-8, 2011/12317-3]
- Coordenagao de Aperfeigoamento de Pessoal de Nivel Superior (CAPES, Office for the Advancement of Higher Education)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, The National Council for Scientific and Technological Development) [402508/2012-2]
- NAPTOXAN-USP
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/13590-8] Funding Source: FAPESP
The role of diet in venom composition has been a topic of intense research interest. This work presents evidence that the variation in the venom composition from the scorpion Tityus serrulatus (Ts) is closely associated with post-starvation extraction time and prey-specific diet. The scorpions were fed with cockroach, cricket, peanut beetle or giant Tenebrio. The venoms demonstrated a pronounced difference in the total protein and toxins composition, which was evaluated by electrophoresis, reversed-phase chromatography, densitometry, hyaluronidase activity and N-terminal sequencing. Indeed, many toxins and peptides, such as Ts1, Ts2, Ts4, Ts5, Ts6, Ts15, Ts19 frag. II, hypotensins 1 and 3, PAPE peptide and peptide 9797 (first described in Ts venom), were all identified in different proportions in the analyzed Ts venoms. This study is pioneer on assessing the influence of the starvation time and the prey diet on hyaluronidase activity as well as to describe a modification of Tricine-gel-electrophoresis to evaluate this enzyme activity. Altogether, this study reveal a large contribution of the extraction time and diet on Ts venom variability as well as present a background to recommend the cockroach diet to obtain higher protein content and the cricket diet to obtain higher hyaluronidase specific activity. (C) 2014 Elsevier Ltd. All rights reserved.
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