期刊
TOXICON
卷 60, 期 7, 页码 1223-1227出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2012.08.018
关键词
Tarantula; Venom; Brachypelma vagans; Hyaluronidase; Baculovirus; Recombinant protein
资金
- DGAPA-UNAM [IN230203]
Hyaluronidases (Hyal) present in the venom of poisonous animals have been considered as spreading factors that facilitate a fast penetration of the venom in the prey. We have found that hyaluronidase from the tarantula Brachypelma vagans venom (BvHyal) displays a substrate-specific Hyal activity against hyaluronan. By using a combined strategy based on peptide sequencing and RT-PCR, we have cloned a BvHyal cDNA. Active recombinant BvHyal was efficiently expressed in a baculovirus system in insect cell. (C) 2012 Elsevier Ltd. All rights reserved.
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