Identification, cDNA cloning and heterologous expression of a hyaluronidase from the tarantula Brachypelma vagans venom

Hyaluronidases (Hyal) present in the venom of poisonous animals have been considered as spreading factors that facilitate a fast penetration of the venom in the prey. We have found that hyaluronidase from the tarantula Brachypelma vagans venom (BvHyal) displays a substrate-specific Hyal activity against hyaluronan. By using a combined strategy based on peptide sequencing and RT-PCR, we have cloned a BvHyal cDNA. Active recombinant BvHyal was efficiently expressed in a baculovirus system in insect cell. (C) 2012 Elsevier Ltd. All rights reserved.