期刊
PLOS PATHOGENS
卷 11, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.ppat.1004660
关键词
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资金
- Ministry of Education ERC CZ grant [LL1205]
- Czech Grant Agency [P302/12/2513]
- EMBO [1965]
- Grant Agency of University of South Bohemia [140/2010/P, 04/092/2011/P]
- European Union [316304]
In the infectious stage of Trypanosoma brucei, an important parasite of humans and livestock, the mitochondrial (mt) membrane potential (Delta psi(m)) is uniquely maintained by the ATP hydrolytic activity and subsequent proton pumping of the essential FoF1-ATPase. Intriguingly, this multiprotein complex contains several trypanosome-specific subunits of unknown function. Here, we demonstrate that one of the largest novel subunits, ATPaseTb2, ismembrane-bound and localizes with monomeric and multimeric assemblies of the FoF1-ATPase. Moreover, RNAi silencing of ATPaseTb2 quickly leads to a significant decrease of the Delta psi(m) that manifests as a decreased growth phenotype, indicating that the FoF1-ATPase is impaired. To further explore the function of this protein, we employed a trypanosoma strain that lacks mtDNA (dyskinetoplastic, Dk) and thus subunit a, an essential component of the proton pore in the membrane F-o-moiety. These Dk cells generate the Delta psi(m) by combining the hydrolytic activity of the matrix-facing F-1-ATPase and the electrogenic exchange of ATP(4)- for ADP(3)- by the ATP/ADP carrier (AAC). Surprisingly, in addition to the expected presence of F-1-ATPase, the monomeric and multimeric FoF(1)-ATPase complexes were identified. In fact, the immunoprecipitation of a F-1-ATPase subunit demonstrated that ATPaseTb2 was a component of these complexes. Furthermore, RNAi studies established that the membrane-bound ATPaseTb2 subunit is essential for maintaining normal growth and the Delta psi(m) of Dk cells. Thus, even in the absence of subunit a, a portion of the FoF1-ATPase is assembled in Dk cells.
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