期刊
TOXICON
卷 55, 期 4, 页码 897-903出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2009.12.019
关键词
Microcystins; Benthic cyanobacteria; 16S rRNA; Planktothrix; Phormidium; New Zealand
资金
- Victoria University of Wellington strategic research fund
- New Zealand Foundation for Research Science and Technology (FRST) [CAWX0703]
- Andy Ritchie Memorial Trust scholarship
- FRST post-doctoral fellowship [FRST post-doctoral fellowship (CAWX0501]
In November 2008 a dog died soon after ingesting benthic algal mat material from the Waitaki River, New Zealand. Based on a morphological examination of environmental material, the causative organism was putatively identified as the filamentous cyanobacterium Phormidium sp. Two strains (VUW25 and CYN61) were isolated and cultured to enable further taxonomic and cyanotoxin characterisation. Phylogenetic analyses based on a region of the 16S rRNA gene sequence, intergenic spacer (ITS) region and the mcyE gene demonstrated that the species was likely to be a new Planktothrix species that is either benthic or has a biphasic life cycle. Using liquid chromatography-mass spectrometry (LC-MS), microcystin-LR, [D-Asp(3), Dha(7)] microcystin-LR, [D-Asp(3)] microcystin-LR, and minor proportions of [D-Asp(3), ADMAdda(5)] microcystin-LhR were identified. This is the first report of [D-Asp(3)] microcystin-LR, [D-Asp(3), Dha(7)] microcystin-LR and an ADMAadda variant in New Zealand. No cylindrospermopsins, saxitoxins or anatoxins were detected. Dog deaths caused by the consumption of cyanobacterial mats containing anatoxins have previously been reported in New Zealand. To our knowledge, however, this is the first instance of a benthic microcystin-producing species causing an animal death in New Zealand. (C) 2009 Elsevier Ltd. All rights reserved.
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