期刊
TOXICON
卷 56, 期 3, 页码 474-479出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2010.04.009
关键词
Scorpion venom hyaluronidase; Full protein sequence; 3 ' RACE PCR; Edman degradation; Phylogenetic tree
资金
- DSO-NUS [R-181-000-123-592]
In this communication, the full protein sequence of a novel venom hyaluronidase BmHYA1 was reported. It is the first full hyaluronidase amino acid sequence from scorpion venom. It was deduced from nucleotide sequence by 3' Rapid Amplification of cDNA Ends (RACE) PCR cloning, followed by alignment with the N-terminal amino acid sequence, which was obtained by Edman degradation. BmHYA1 has 385 amino acid residues containing five potential N-glycosylation sites. The phylogenetic analysis indicates early divergence and independent evolution of BmHYA1 from other hyaluronidases. (C) 2010 Elsevier Ltd. All rights reserved.
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