4.4 Article

Identification and characterization of phospholipase A2 inhibitors from the serum of the Japanese rat snake, Elaphe climacophora

期刊

TOXICON
卷 53, 期 6, 页码 685-692

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2009.02.001

关键词

Phospholipase A(2); PLA(2); Phospholipase A(2) inhibitor; PLI; Colubridae; cDNA sequence

资金

  1. Ministry of Education, Science, Sports, and Culture of Japan [12672137]
  2. Grants-in-Aid for Scientific Research [12672137] Funding Source: KAKEN

向作者/读者索取更多资源

Two distinct phospholipase A(2) (PLA(2)) inhibitory proteins (PLIs) were purified from the serum of the Japanese rat snake, Elaphe climacophora. The 150-kDa inhibitor, a trimer of a 50-kDa subunit, specifically inhibited the basic PLA(2) purified from the venom of Gloydius brevicaudus, whereas the 120-kDa one composed of two distinct 25-kDa subunits, A and B, inhibited both the acidic and basic PLA(2)S of G. brevicaudus. On the basis of their amino acid sequences, these inhibitors were assigned as PLI beta and PLI gamma, respectively. A PLI alpha homolog (PLI alpha-like protein; PLI alpha-LP) having an apparent molecular weight of 50-kDa and composed of 15-kDa subunits was also purified from the E climacophora serum. This homolog was immunoreactive with antibody raised against the G. brevicaudus PLI alpha, but lacked in the inhibitory activity toward the acidic and basic PLA(2)s. The cDNAs encoding PLI alpha-LP, PLI beta, PLI gamma-A, and PLI gamma-B were cloned from liver RNA, and their nucleotide sequences were compared with those of other venomous and non-venomous snakes. (C) 2009 Elsevier Ltd. All rights reserved.

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