期刊
TOXICON
卷 51, 期 5, 页码 774-779出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2007.12.003
关键词
tetrodotoxin (TTX); Nassarius; liquid chromatography-mass spectrometry (LC-MS); selected ion monitoring (SIM)
Tetrodotoxin (TTX) has been usually analyzed in marine and other sources by HPLC separation, followed by alkaline treatment and fluorescence assay. However, the applications of such methods were limited because of their tedious extraction and derivation procedures. A simpler, rapid LC-MS method, using selected ion-monitoring, has now been developed, and used to identify and quantitate TTX in a marine gastropod of the genus Nassarius, collected from Fijian province of China. The toxin was confirmed comparable to authentic TTX by NIS and MSn. In addition, a moderate amount of TTX was detected in the Nassarius by this novel approach, suggesting that Nassarius would be a potential food poison source. (c) 2007 Elsevier Ltd. All rights reserved.
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