期刊
TOXICOLOGY MECHANISMS AND METHODS
卷 20, 期 7, 页码 424-427出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/15376516.2010.490968
关键词
Paraquat; urine samples; enzymatic-spectrophotometric method; redox cycling
类别
资金
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [05/51592-9]
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
Paraquat is a broad-spectrum contact herbicide that has been encountered worldwide in several cases of accidental, homicidal, and suicidal poisonings. The pulmonary toxicity of this compound is related to the depletion of NADPH in the pneumocytes, which is continuously consumed by the reduction/oxidation of paraquat and reductase enzyme systems in the presence of O-2 (redox cycling). Based on this mechanism, an enzymatic-spectrophotometric method was developed for the determination of paraquat in urine samples. The velocity of NADPH consumption was monitored at 340 nm, every 10 s during 15 min. The velocity of NADPH oxidation correlated with the paraquat levels found in samples. The enzymatic-spectrophotometric method showed to be sensitive, making possible the detection of paraquat in urine samples at concentrations as low as 0.05 mg/L.
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