期刊
TOXICOLOGY LETTERS
卷 224, 期 2, 页码 246-256出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2013.10.027
关键词
Nitric oxide; Muscarinic receptor; Silver nanoparticles; Airway; Proliferation; Apoptosis
类别
资金
- Integral Program for Institutional Support [C10-PIFI-03.02.02]
- National Council of Science and Technology of Mexico [407087]
Silver nanoparticles (AgNPs) are used to manufacture materials with new properties and functions. However, little is known about their toxic or beneficial effects on human health, especially in the respiratory system, where its smooth muscle (ASM) regulates the airway contractility by different mediators, such as acetylcholine (ACh) and nitric oxide (NO). The aim of this study was to evaluate the effects of AgNPs on ASM cells. Exposure to AgNPs induced ACh-independent expression of the inducible nitric oxide synthase (iNOS) at 100 mu g/mL, associated with excessive production of NO. AgNPs induced the muscarinic receptor activation, since its blockage with atropine and blockage of its downstream signaling pathway inhibited the NO production. AgNPs at 10 and 100 mu g/mL induced ACh-independent prolonged cytotoxicity and decreased cellular proliferation mediated by the muscarinic receptor-iNOS pathway. However, the concentration of 100 mu g/mL of AgNPs induced muscarinic receptor-independent apoptosis, suggesting the activation of multiple pathways. These data indicate that AgNPs induce prolonged cytotoxic and anti-proliferative effects on ASM cells, suggesting an activation of the muscarinic receptor-iNOS pathway. Further investigation is required to understand the full mechanisms of action of AgNPs on ASM under specific biological conditions. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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