期刊
TOXICOLOGY LETTERS
卷 223, 期 1, 页码 16-24出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2013.08.017
关键词
Potassium channel; Patch clamp; Silica nanoparticle; Human vascular endothelial cells; Inflammation
类别
资金
- National Natural Science Foundation of China [81001236]
Exposure to nanoparticles became popular in industry and daily life. Nano-SiO2 was shown to have an adverse effect to vascular endothelial cell although the mechanisms remain unclear. To test whether the nano-SiO2' s harmful effect was related to the potassium channel, human umbilical vascular endothelial cells (HUVECs) were treated with nano-SiO2 in different dose. Cell survival rate and lactate dehydrogenase (LDH) as cytotoxic parameters, tumor necrosis factor-alpha(TNF-alpha) and interleukin-6 (IL-6) as inflammation indicators were determined. The electrophysiological changes and function of potassium channel were detected with patch clamp and channel blockers. It was found that nano-SiO2 exposure decreased cell survival rate, increased LDH leakage, TNF-alpha and IL-6 production. The potassium channel activity was increased in the opening rate and current intensity. Furthermore, potassium channel blockers tetraethylammonium (TEA), 4-amino pyridine (4-AP), and margatoxin (MGTX) reduced the nano-SiO2-induced cytotoxity and inflammation, i.e., increase in the cell survival rate, and decrease in the LDH leakage and production of TNF-alpha and IL-6. It might be concluded that the nano-SiO2-induced inflammation and cytotoxicity at HUVECs was associated with the activation of potassium channel. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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