期刊
TOXICOLOGY LETTERS
卷 214, 期 2, 页码 175-181出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2012.08.027
关键词
Hepatotoxicity; High-content screening; Real-time monitoring; Mitochondria dysfunction; Apoptosis; Cytosolic calcium
类别
资金
- National Research Foundation of Korea (NRF) Grant
- Ministry of Education, Science and Technology (MEST) [2012-0005653]
- Korea Healthcare Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea [A100096]
A quantitative high-content screening (HCS) was suggested for the real-time monitoring of drug-induced mitochondrial dysfunction-mediated hepatotoxicity. This HCS is very advantageous in that it allows simultaneous observation of drug-induced activations of hepatotoxic pathways using hypermulticolor cellular imaging. The mitochondrial permeability transition (MPT), cytosolic calcium, and caspase-3 were selected as functional markers to verify drug-induced hepatotoxicity and were concurrently monitored in HepG2 cells in a real-time manner. Nefazodone, tolcapone, and troglitazone caused mitochondrial dysfunction and subsequent apoptotic HepG2 cell death in addition to marked cytosolic calcium increase. On the other hand, extrinsic pathway-mediated apoptotic cell death was monitored when HepG2 cells were treated with piroxicam. It was found that piroxicam-treated HepG2 cells showed apoptotic cell death without the MPT formation, while a cytosolic calcium increase was clearly observed. This finding was confirmed by the caspase-8 inhibition assay. These results demonstrated the unique potential of real-time hypermulticolor HCS to screen hepatotoxic drugs at the in vitro stage rather than the later in vivo stage based on an animal model and to ultimately reduce the probability of drug failure. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
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