期刊
TOXICOLOGY LETTERS
卷 210, 期 3, 页码 285-292出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2012.02.014
关键词
Polycyclic aromatic hydrocarbons (PAHs); Primordial germ cells; Gametogenesis; Meiosis; Aromatic hydrocarbon receptor (AHR)
类别
资金
- National Basic Research Program of China (973 program) [2011CB111513]
- National Science Foundation of China [31101884]
- Zhejiang Provincial Science and Technology Department [2011R09031-03]
- Ministry of Education [2007U07121]
Numerous studies have demonstrated that polycyclic aromatic hydrocarbon (PAH) exposure during pregnancy is associated with negative reproductive consequences in adulthood, such as reduced sperm production and oocyte destruction in human and laboratory animals. However, the mechanism(s) underlying these epidemiological findings is still unclear, largely due to the lack of an in vitro model of gametogenesis. Here we established an in vitro model of gametogenesis through retinoic acid (RA)induced differentiation of chicken primordial germ cells (PGCs) that progressed through meiosis to generate 55% of haploid cells. Using this system, we demonstrated that PAH exposure directly suppressed meiotic initiation and completion in chicken PGCs and promoted the apoptosis of PGCs, eventually leading to reduction of haploid cell formation by 66.9%. Furthermore, knockdown experiments suggested that AHR was essential for the adverse effects of PAH on PGC differentiation. Collectively, these findings offer novel insight into the dynamic mechanism by which PAH affects gametogenesis, suggesting that AHR-dependent inhibition of meiosis might be the cause of reduced gamete production. This study also provides a novel model for direct examination of the molecular and genetic pathways of environmental toxicants on gametogenesis. Crown Copyright (C) 2012 Published by Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据