期刊
TOXICOLOGY LETTERS
卷 205, 期 1, 页码 86-95出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2011.05.1018
关键词
Gold nanoparticles; Glutathione depletion; Hydrogen peroxide generation; Apoptosis
类别
资金
- National Basic Research Program of China (973 Program) [2007CB936000]
- National Natural Science Funds for Distinguished Young Scholar [20725518]
- National Key Natural Science Foundation of China [21035003]
- Science and Technology Development Programs of Shandong Province of China [2008GG30003012]
- Key Natural Science Foundation of Shandong Province of China [ZR2010BZ001]
Gold nanoparticles (AuNPs) have shown promising biological and military applications due to their unique electronic and optical properties. However, little is known about their cytotoxicity when they come into contact with a biological system. The primary objective of this study is to determine the sequence of apoptotic signaling events that occur after modulation of the cellular redox state in HL7702 cells (human liver cell line), with emphasis on the role of the interaction of AuNPs with glutathione (GSH). After incubation with 8 nm AuNPs at 50 nM, there was an early decline in cytosolic GSH. which initiated mitochondrial transmembrane potential (Delta psi m) depolarization and apoptosis. Mitochondrial GSH depletion was observed at approximately 48 h, after which mitochondrial hydrogen peroxide (H2O2) production increased significantly and apoptosis was further exacerbated. Bax translocation, cytochrome c release and downstream caspase 3 were first detected at 24 h, notably after 48 h, corresponding with increasing H2O2 level. These data suggest that HL7702 cells are depleted of intracellular GSH as a result that 8 nm AuNPs possess strong Au-S bonding interactions with GSH. A decrease in GSH alone can act as a potent early activator of apoptotic signaling. Increased H2O2 production following mitochondrial GSH depletion represents a crucial event, which commits HL7702 cells to apoptosis through mitochondrial pathway. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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