期刊
TOXICOLOGY LETTERS
卷 192, 期 3, 页码 324-329出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2009.11.002
关键词
Fluorosis; ERK1/2; Learning and memory; nAChRs; Rats
类别
资金
- Foundation in the China National Natural Scientific Foundation [30760224]
- Foundation in the Ministry of Science and Technology of China [S2010GR0928]
- Government Foundations in Guizhou Province of China
- Foundation of Guiyang Medical College
In order to reveal the mechanism of the decreased ability of learning and memory induced by chronic fluorosis, nicotinic acetylcholine receptors (nAChRs) and the pathway of extracellular signal regulated protein kinase (ERK1/2) were investigated by using the rats fed with different concentrations of sodium fluoride for 6 months Spatial learning and memory of the rats were evaluated by Morris Water Maze test The expressions of nAChRs, ERK1/2 and mitogen-induced extracellular kinase (MEK1/2) at protein and mRNA levels were detected by Western blotting and real-time PCR, respectively. The results showed that as compared with controls, the learning and memory capacity in the rats with fluorosis was decreased The protein expressions of alpha 7 and alpha 4 nAChR subunits in rat brains with fluorosis were decreased by 35% and 33%, whereas the corresponding receptor subunit mRNAs did not exhibit any changes The increases of phospho- and total-ERK1/2 as well as phospho-MEK1/2 at the protein levels were found in the brains of rats with fluorosis as compared to controls, and no difference of ERK1/2 mRNA was found In addition,the activation rate of phospho-ERK1/2 was decreased in the brains affected with fluorosis The modifications of nAChRs and ERK1/2 pathway might be connected with the molecular mechanisms in the decreased capacity of learning and memory of the rats with fluorosis (C) 2009 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据