期刊
TOXICOLOGY LETTERS
卷 199, 期 3, 页码 323-332出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2010.09.015
关键词
Benzo[a]pyrene (B[a]P); Di-(2-ethylhexyl) phthalate (DEHP); Combination; Ovotoxicity; Granulosa cells; Mechanism
类别
资金
- National Natural Science Foundations of China [30630056, 30800900]
- Program of S&T (Chongqing) Project [CSTC2006AA7003]
The aim of the present study was to determine the ovotoxicity of female Sprague-Dawley (SD) rats exposed to benzo[a]pyrene (B[a]P) and di-(2-ethylhexyl) phthalate (DEHP), either alone or in combination; the molecular mechanism and the combined effects were also evaluated. Female rats were given intragastric administration of control (corn oil), B[a]P (5 and 10 mg/kg), DEHP (300 and 600 mg/kg) and B[a]P + DEHP (at 5 mg/kg and 300 mg/kg respectively, or at 10 mg/kg and 600 mg/kg respectively) on alternate days for 60 days. Relative ovary weight, estrous cycle, 17 beta-estradiol blood level, ovarian follicle populations, granulosa cell apoptosis, and gene and protein expression of P450Arom and PPAR were investigated. Our study demonstrated that the combination of B[a]P and DEHP exerts ovotoxicity in female rats and suppression of sex hormone secretion and homeostasis, which is associated with prolonged estrous cycles, decreases in ovarian follicle populations and granulosa cell apoptosis involving a PPAR-mediated signaling pathway of action of the two chemicals. In addition, based on qualitative assessment of the combined toxicity, no interaction effects were observed following combined B[a]P and DEHP administration. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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