期刊
TOXICOLOGY LETTERS
卷 191, 期 2-3, 页码 231-235出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2009.09.003
关键词
Methylmercury; Intracellular Zn2+; Cellular thiol
类别
资金
- Office of Dean
- Faculty of Integrated Arts and Sciences
- The University of Tokushima, Tokushima, Japan
We have previously revealed that thimerosal. an organomercurial preservative, increases intracellular Zn2+ concentration in rat thymocytes. Because thimerosal contains ethylmercury that confers the toxicity, it is a possibility that methylmercury (MetHg). an environmental pollutant, also increases intracellular Zn2+ concentration This possibility was tested by measuring intracellular Zn2+ level with FluoZin-3. a fluorescent probe for intracellular Zn2+. MetHg at concentrations ranging from 100 nM to 1 mu M significantly increased the intensity of FluoZin-3 fluorescence, an indicator for intracellular Zn2+ concentration, under external Ca2+- and Zn2+-free condition in a concentration-dependent manner. TPEN, a chelator for intracellular Zn2+, completely diminished the MetHg-induced augmentation of FluoZin-3 fluorescence MetHg at 100 nM or more significantly decreased the intensity of 5-chlormethylfluorescein fluorescence, an indicator for cellular thiol content Such MetHg-induced changes in the fluorescence were correlated with a coefficient of -0.917. Taken together, it is suggested that submicromolar MetHg releases Zn2+ from intracellular thiol, resulting in the increase in intracellular Zn2+ concentration. However, it is unlikely that MetHg at critical maternal blood concentration (27 nM) affects intracellular Zn2+ homeostasis (C) 2009 Elsevier Ireland Ltd All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据