期刊
TOXICOLOGY LETTERS
卷 191, 期 2-3, 页码 236-239出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2009.09.005
关键词
delta-Aminolevulinic acid dehydratase (ALAD); Ethnic variation; Africans; Asians; Mexicans; Polymorphism
类别
资金
- Grants-in-Aid for Scientific Research [21590736] Funding Source: KAKEN
delta-Aminolevulinic acid dehydratase (ALAD) is a cytosolic enzyme in the heme biosynthetic pathway. The ALAD is controlled by two codominant alleles (ALAD1 and ALAD2), which result in a Asn-Lys substitution at amino acid position 59 of the mature enzyme based on a single nucleotide polymorphism (SNP)(G177C) leading three phenotypes (ALAD1-1, ALAD1-2, and ALAD2-2). Previous studies have shown that this polymorphism is related to lead toxicity. There is little evidence showing interethnic differences in the distribution of this polymorphism We examined the distribution of genetic variants of the ALAD G177C polymorphism in four Asians, three Africans, and three Mexicans. Genomic DNA was extracted from blood or bloodstain, and the genotypes for the ALAD polymorphism were determined by PCR followed by RFLP digestion and gel electrophoresis. We found a notable interethnic disparity in the distribution of ALAD G177C genotypes and alleles The frequencies of ALAD2 in Asian populations were comparable to those in Caucasians. while Africans had no mutation allele. These findings may help us understand the interethnic disparities in susceptibility to lead toxicity. (C) 2009 Elsevier Ireland Ltd. All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据