期刊
TOXICOLOGY LETTERS
卷 177, 期 2, 页码 116-122出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2008.01.003
关键词
hexachlorobenzene; AhR; c-Src kinase; rat liver; EGFR-Tyr845; WB-F344 cells
类别
Hexachlorobenzene (HCB) is a widespread environmental pollutant. It has some properties that are typical for dioxin-like compounds that act mainly through the aryl hydrocarbon receptor (AhR) protein. Upon dioxin binding, the AhR translocates to the nucleus and modulates gene expression. At the same time, c-Src kinase frees from the AhR complex and thereby activates its own kinase activity, which acts as a trigger for the growth factor receptor signal transduction pathway. HCB is a weak agonist of the AhR, and the evidence that HCB toxicity is mediated via the AhR complex is limited and inconclusive. In the present study, female Wistar rats were administered HCB (1, 10 and 100mg/kg)for 30 days. Liver cytosolic AhR was translocated to the nucleus. The activity of liver microsomal c-Src increased at all assayed doses. HCB induced the association of the EGFR with c-Src and increased the phosphorylation of EGFR at tyrosine 845 (Tyr845), a known c-Src phosphorylation site. c-Src from WB-F344 cells treated with HCB exhibited increased protein levels and c-Src-pTyr416 phosphorylation than the control cells. Again HCB induced EGFR phosphorylation at Tyr845. Such an effect of HCB could not be detected when c-Src activity was blocked by PP2. All together, our data demonstrates that HCB may induce EGFR transactivation through an c-Src-dependent pathway. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据