期刊
TOXICOLOGY IN VITRO
卷 28, 期 8, 页码 1424-1429出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2014.07.009
关键词
Bisphenol A; Primordial follicle pool; Over-activation; PI3K/Akt pathway
类别
资金
- National Natural Science Foundation of China [81272213/H1602]
- Major Scientific Projects for Army [13CXZ006]
The worldwide increase in the use of bisphenol A (BPA) has resulted in increased human exposure, which could affect human reproductive function. Few studies have investigated the effect of BPA exposure on the primordial follicle pool. In this study, we employed a neonatal ovarian culture system comprising organ obtained from female C57BL/6 pups on postnatal day 4 to assess the effect of EPA on the primordial follicle pool. Ovaries were cultured with BPA (0.1 mu M, physiological concentration found in children's blood, and 1 mu M, 10 mu M) or vehicle for 10 days. Our study revealed that the primary follicle number increased during the early time points (<= 5 days), and we observed a reduction in the primordial follicle pool at a later time point (day 10). This reduction at day 10 was due to increased follicle activation and reduced follicle atresia, as determined by immunohistochemistry for Ki-67 and active caspase-3. Then we examined the phosphatidylinositol-3-kinase (PI3K)/Akt pathway, which is known to be important for early follicle growth. BPA exposure induced the upregulation of the PI3K/Akt pathway, which was reversed by concomitant treatment with PI3K inhibitor. Our results reveal a novel mechanism for BPA-induced primordial follicle activation that involves the PI3K/Akt pathway. (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据