期刊
TOXICOLOGY IN VITRO
卷 27, 期 6, 页码 1962-1969出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2013.06.012
关键词
1-Desulfoyessotoxin; Yessotoxin; Paraptosis; Cytoplasmic vacuolation; Mitochondria; Endoplasmic reticulum; DNA fragmentation; p38; Hsp70; Intracellular signalling
类别
资金
- Norwegian School of Veterinary Science, Department of Basic Sciences and Aquatic Medicine and Olav Raagholt og Gerd Meidel Raagholts legacy
- New Zealand Foundation for Research, Science and Technology [AGRX0402]
- Marie Curie International Incoming Fellowship
- European Community [221117]
1-Desulfoyessotoxin (1-dsYTX) is a desulfated polyether compound belonging to the yessotoxin group of marine toxins. This analogue has been detected in mussels. There are so far no reports on the mechanisms of action of 1-dsYTX in in vitro cell systems. This work evaluates cytotoxic responses in BC3H1 cells exposed to 100 nM 1-dsYTX. The toxicity of 1-dsYTX seems to be similar to that of yessotoxin (YTX). 1-Desulfoyessotoxin induced morphological and biochemical traits typical of a non-apoptotic form of cell death resembling paraptosis. Treated BC3H1 cells showed extensive cytoplasmic vacuolation, enlargement of mitochondria and endoplasmic reticulum and lack of DNA fragmentation. Western blotting analysis revealed phosphorylation of the protein kinase p38 and involvement of the heat shock protein Hsp70. This activation suggests involvement of different signalling pathways for programmed cell death. (C) 2013 Elsevier Ltd. All rights reserved.
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