期刊
TOXICOLOGY IN VITRO
卷 26, 期 7, 页码 1122-1128出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2012.06.015
关键词
Resveratrol; Intestinal cells; Lipopolysaccharide; COX-2; PGE(2); NF-kappa B
类别
资金
- Dottorato di ricerca in Morfobiologia Applicata e Citometabolismo dei Farmaci, XXII ciclo
Resveratrol is a natural phytoalexin present in a variety of plant species, such as grapes and red wine, that is well known for its anti-inflammatory effects. In addition, a cancer chemotherapeutic activity of resveratrol has been described. Here we evaluated the effect of resveratrol on COX-2 and prostaglandin E-2 production in human intestinal cells Caco-2 cells treated with lipopolysaccharide (LPS). Resveratrol concentration-dependently inhibited the expression of COX-2 mRNA in the LPS-treated cells, as well as protein expression, resulting in a decreased production of PGE(2). In order to investigate the mechanisms through which resveratrol exhibited these anti-inflammatory effects, we examined the activation of I kappa B in LPS-stimulated intestinal cells. Results demonstrated that resveratrol inhibited the translocation of NF-kappa B subunits from the cytosol to the nucleus, which correlated with its inhibitory effects on lact phosphorylation and degradation. These results suggest that the down-regulation of COX-2 and PGE(2) by resveratrol may be related to NF-kappa B inhibition through the negative regulation of IKK phosphorylation in intestinal cells. (C) 2012 Elsevier Ltd. All rights reserved.
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