期刊
TOXICOLOGY IN VITRO
卷 24, 期 5, 页码 1356-1362出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2010.04.009
关键词
Gallic acid; Lung cancer cell; ROS; GSH; NAC
类别
资金
- Ministry of Education, Science and Technology [20100007059]
Gallic acid (GA) widely distributed in plants and foods has its various biological effects. Here, we investigated the anti-cancer effects of GA on Calu-6 and A549 lung cancer cells in relation to reactive oxygen species (ROS) and glutathione (GSH). GA dose-dependently decreased the growth of Calu-6 and A549 cells with an IC50 of approximately 10-50 mu M and 100-200 mu M GA at 24 h, respectively. GA also induced cell death in lung cancer cells, which was accompanied by the loss of mitochondrial membrane potential (MMP; Delta Psi(m)). The percents of MMP (Delta Psi(m)) loss and death cells were lower in A549 cells than Calu-6 cells. GA increased ROS levels including O-2(center dot-) in lung cancer cells at 24 h and also GSH depleted cell numbers at this time. N-acetyl-cysteine (NAC; a well-known antioxidant) intensified growth inhibition and death in GA-treated lung cancer cells. NAC changed ROS levels and increased GSH depletion in these cells. Vitamin C significantly attenuated cell death. ROS levels and GSH depletion in GA-treated lung cancer cells. L-buthionine sulfoximine (BSO; an inhibitor of GSH synthesis) slightly enhanced growth inhibition and death in GA-treated lung cancer cells and also mildly increased ROS levels and GSH depletion in these cells. In conclusion, GA inhibited the growth of lung cancer cells. GA-induced lung cancer cell death was related to GSH depletion as well as ROS level changes. (C) 2010 Elsevier Ltd. All rights reserved.
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