期刊
TOXICOLOGY IN VITRO
卷 24, 期 7, 页码 1979-1985出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2010.08.014
关键词
Reporter gene assay; Pesticides; Androgen receptor; Glucocorticoid receptor; Anti-androgenic potency
类别
资金
- French Ministry of Ecology and Sustainable Development [P189-DRC03-08]
Pesticides have been suspected to act as endocrine disruptive compounds (EDCs) through several mechanisms of action, however data are still needed for a number of currently used pesticides. In the present study, 30 environmental pesticides selected from different chemical classes (azole, carbamate, dicarboximide, organochlorine, organophosphorus, oxadiazole, phenylureas, pyrazole, pyrimidine, pyrethroid and sulfonylureas) were tested for their ability to alter in vitro the transcriptional activity of the androgen receptor in the MDA-kb2 reporter cell line. The responsiveness of the system was checked by using a panel of reference ligands of androgen and glucocorticoid receptors. When tested alone at concentrations up to 10 mu M, none of the studied pesticides were able to induce the reporter gene after a 18 h exposure. Conversely, co-exposure experiments with 0.1 nM dihydrotestosterone (DHT) allowed identifying 15 active pesticides with IC(50) ranging from 0.2 mu M for vinclozolin to 12 mu M for fenarimol. Fipronil and bupirimate were here newly described for their AR antagonistic activity. (C) 2010 Elsevier Ltd. All rights reserved.
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