期刊
TOXICOLOGY IN VITRO
卷 23, 期 6, 页码 1092-1099出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2009.05.020
关键词
Thimerosal; Intracellular zinc; Oxidative stress
类别
Thimerosal (TMR), an ethylmercury-containing preservative in pharmaceutical products, was recently reported to increase intracellular Zn2+ concentration. Therefore, some health concerns about the toxicity of TMR remain because of physiological and pathological roles of Zn2+. To reveal the property of TMR-induced increase in intracellular Zn2+ concentration, the effect of TMR on FluoZin-3 fluorescence, an indicator of intracellular Zn2+, of rat thymocytes was examined. TMR at concentrations ranging from 0.3 mu M to 10 mu M increased the intensity of FluoZin-3 fluorescence in a concentration-dependent manner under external Ca2+ and Zn2+-free condition. The threshold concentration was 0.3-1 mu M. The increase in the intensity was significant when TMR concentration was 1 mu M or more. N,N,N',N'-Tetrakis(2-pyridlylmethyl)ethylenediamine (TPEN), a chelator for intracellular Zn2+, completely attenuated the TMR-induced augmentation of FluoZin-3 fluorescence. Hydrogen peroxide (H2O2) and N-ethylmaleimide, reducing cellular thiol content, significantly increased FluoZin-3 fluorescence intensity and decreased 5-chloromethylfluorescein (5-CMF) fluorescence intensity, an indicator for cellular thiol. The correlation coefficient between TMR-induced augmentation of FluoZin-3 fluorescence and attenuation of 5-CMF fluorescence was -0.882. TMR also attenuated the 5-CMF fluorescence in the presence of TPEN. Simultaneous application of H2O2 and TMR synergistically augmented the FluoZin-3 fluorescence. It is suggested that TMR increases intracellular Zn2+ concentration via decreasing cellular thiol content. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据